Ushakova N.A.

А.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences

INFLUENCE OF PROBIOTICS IN THE COMPOSITION OF FEED SUBSTRATE ON THE AMINO ACID PROFILE OF HERMETIA ILLUCENS LARVES

Abstract
The use of probiotics Bacillus subtilis B-8130 and Bacillus licheniformis B-4162 as part of the feed mixture in the cultivation of larvae of the black soldier fly Hermetia illucens influenced the features of the amino acid profile of the larvae. The greatest impact was caused by B. licheniformis B-4162, which caused a statistically significant decrease in the level of 10 of the 18 essential amino acids, including the sulfur-containing amino acids cystine and methionine. B subtilis B-8130 demonstrated an unreliable tendency to decrease the total amino acid content, but statistically significantly reduced the cystine content compared to the control.
The revealed changes in the amino acid content in the larvae occurred against the background of an increase in their mass compared to the control larva. It is assumed that the decrease in the level of amino acids is associated with their expenditure on the formation of glucose and metabolic energy in a more intensively developing larva on a feed mixture with a probiotic.
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PURIFICATION OF WASTE WATER FROM THE PRODUCTION OF LIPID CONCENTRATE OF HERMETIA ILLUCENS GARVES WITH WASTE FROM PROCESSING OF BIOMASS PISUM SATIVUM

Abstract
The possibility of using the crushed biomass of the pods of the common pea (Pisum sativum) as a sorption material for purifying model waters from the production of lipid concentrate from the larvae of the fly of the species Hermetia illucens has been investigated. Information on the composition and amount of lipids and saturated and unsaturated fatty acids in insect larvae is briefly presented. The effect of the particle size of the sorption material on the efficiency of the adsorptive extraction of the lipid concentrate emulsion from the model wastewater was investigated and an increase in the degree of purification with an increase in the dispersion of the dried biomass of pea pods was revealed. The dependence of the efficiency of removal of emulsion particles on the dosage of the sorption material and the time of contact of the latter with the sorbate was also investigated. The optimal pa-rameters of adsorption purification have been determined.
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