INTEGRAL ASSESSMENT OF WATER ENVIRONMENT TREATED WITH ZOOCOMPOST WITH THE USE OF ALLIUM SULFUR

, ,
 5-14 p.
An integral assessment of the state of aquatic environments using biotests is understood as the study of the complex effect of a number of factors such as chemical, physical and biological - on the functions of living organisms. Traditional methods of chemical and physical analysis of the state of various environments (water, air, soil) are based on comparing the results obtained with environmental standards, such as MPC (maximum permissible concentration) or MAL (maximum allowable level). The advantage of biotesting in comparison with other methods for assessing the state of environments is the speed of obtaining the results and their clarity, the absence of expensive reagents and equipment. Organisms used for biotesting are called biotests or bioindicators. When choosing bioindicators the sensitivity of the organism to exposure factors is taken into account as well as the ease of cultivation. Living organisms from different taxonomic groups can act as bioindicators. Such a bioindicator recognized by the International Program for Chemical Safety (IPCS) is the higher plant Allium sulfur L. The Allium test was used to assess aquatic environment treated with zoo compost resulting from the cultivation of the Black Soldier fly larva (Hermetia illucens). The length of the roots of the tested plant was chosen as the primary response to the effect of a complex of factors. The effect of exposure is considered to be established if there is an increase or decrease in the growth of the roots of the tested plants in comparison with the control. The results obtained can be used to develop recommendations for the use of zoo compost.
Keywords:
integral assessment of environments, biotesting, bioindicator, allium test, zoo compost, Black Soldier fly
Porozhnyuk L.A.
Candidate of Engineering Sciences (Ph.D.), Associate Professor, Belgorod State Technological University named after V.G. Shukhov
Lupandina N.S.
Candidate of Engineering Sciences (Ph.D.), Associate Professor, Belgorod State Technological University named after V.G. Shukhov
Nepomenko A.V.
Belgorod State Technological University named after V.G. Shukhov
1. Ingham E.R. Zavarivanie kompostnogo Chaya. Ogorodnik. 2000. № 29. S. 16 – 19.
2. Ingham E.R. Rukovodstvo po zavarivaniyu kompostnogo chaya. Institut ustojchivyh issledovanij, YUdzhin, OR. 2000. 60 s.
3. Zaller J.G. Vnekornevoe opryskivanie ekstraktami vermikomposta: vliyanie na kachestvo plodov i pokazaniya k podavleniyu fitoftoroza polevyh tomato. Biologicheskoe sel'skoe hozyajstvo i sadovodstvo. 2006. № 24 (2). S. 165 – 180.
4. Al'-Mugrabi K.I. Podavlenie fitoftory infestans v kartofele putem vnekornevogo vneseniya pishchevyh pitatel'nyh veshchestv i kompostnogo Chaya. Avstralijskij zhurnal fundamental'nyh i prikladnyh nauk. 2007. № 1 (4). S. 785 – 792.
5. CHzhan W., Han D.Y., Dick W.A., Davis K.R., Hoitink H.A.J. Kompost i kompostnyj vodnyj ekstrakt-inducirovannaya sistemnaya priobretennaya rezistentnost' u ogurca i arabidopsisa. Fitopatologiya. 2007. № 8 (5). S. 450 – 455.
6. Al'-Mugrabi K.I., Bertelemi K., Livingston T., Burgojn A., Puar'e R., Vikram A. Aerobnyj kompost chaj, kompost i sochetanie togo i drugogo umen'shayut vyrazhennost' parshi obyknovennoj (Streptomyces scabiei) na klubnyah kartofelya. ZHurnal nauk o rasteniyah. 2008. № 3. S. 168 – 175.
7. Kerkeni A., Daami-Remadi M., Tarchun N., Hedher M.B. Vliyanie bakterial'nyh izolyatov, poluchennyh iz ekstraktov komposta navoza zhivotnyh, na razvitie fusarium oxysporum f. sp. Radicis-lycopersici. Aziatskij zhurnal patologii rastenij. 2008. № 2 (1). S. 15 – 23.
8. Larkin R.P. Otnositel'noe vliyanie biologicheskih popravok i sevooborotov na pochvennye mikrobnye soobshchestva i pochvennye bolezni kartofelya. Pochvennaya biologiya i biohimiya. 2008. № 40 (6). S. 1341 – 1351.
9. Pendyurin E.A., Rybina S.YU., Smolenskaya L.M. Ispol'zovanie zookomposta CHernoj l'vinki v kachestve organicheskogo udobreniya. Agrarnaya nauka. 2020. № 7-8. S. 106 – 110.
10. Bulgakov N.G. Kontrol' prirodnoj sredy kak sovokupnost' metodov bioindikacii, ekologicheskoj diagnostiki i normirovaniya. Problemy okruzhayushchej sredy i prirodnyh resursov: Obzornaya informaciya. VINITI. 2003. № 4. S. 33 – 70.
11. Bagdasaryan A.S. Effektivnost' ispol'zovaniya test-sistem pri ocenke toksichnosti prirodnyh sred. Ekologiya i promyshlennost' Rossii. 2007. № 1. S. 44 – 48.
12. Ol'kova A.S. Biotestirovanie v nauchno-issledovatel'skoj i prirodoohrannoj praktike Rossii. Uspekhi sovremennoj biologii. 2014. Tom 134. № 6. S. 614 – 622.
13. Hot'ko N.I. Biomonitoring okruzhayushchej sredy v rajonah razmeshcheniya opasnyh promyshlennyh ob"ektov. Teoriya i praktika. Saratov. Izd-vo GosNIIENP. 2015. 184 s.
14. Vitaliev A.B., Turebaev M.N., Elemesova M.SH., Bigalieva R.K. Kul'tura kletok kak test-sistema dlya issledovaniya potencial'noj mutagennoj aktivnosti promyshlennyh zagryaznitelej. Geneticheskie posledstviya zagryazneniya okruzhayushchej sredy. M.: Izd-vo Mysl', 1977. Vyp. 2. S. 74 – 84.
15. Dubinina L.G. Kul'tura lejkocitov cheloveka kak test-sistema pri analize mutagennosti faktorov sredy. Geneticheskie posledstviya zagryazneniya okruzhayushchej sredy. M.: Izd-vo Nauka, 1977. Vyp. 1. S. 89 – 95.
16. Sibircev V.S., Krasnikova L.V., SHlejkin A.G., Stroev S.A., Naumov I.A., Olekhnovich R.O., Tereshchenko V.F., SHabanova E.M., Mussa Al'-Hatib. Novyj metod biotestirovaniya s primeneniem sovremennyh impedansnyh tekhnologij. Nauchno-tekhnicheskij vestnik informacionnyh tekhnologij, mekhaniki i optiki. 2015. № 2. URL: https://cyberleninka.ru/article/n/novyy-metod-biotestirovaniya-s-primeneniem-sovremennyh-impedansnyh-tehnologiy
17. Deryabin D.G., Aleshina E.S. Metodika ekspressnogo opredeleniya toksichnosti pit'evyh mineral'nyh butilirovannyh vod s pomoshch'yu lyuminescentny bakterial'nyh biosensorov. Metodiche-skie rekomendacii FGUZ «Centr gigieny epidemiologii v Orenburgskoj oblasti» ot 27.08. 2007. 24 s.
18. Sazonovec M.A., Ignatenko A.V. Analiz detoksikacii vodnyh sred metodom biotestirovaniya. Trudy BGTU. Minsk. 2014. № 4. S. 179 – 182.
19. Pronina N.A. Biotestirovanie vozdushnogo zagryazneniya benzolom pri pomoshchi kress-salata (Lepidium Sativum). Biodiagnostika sostoyaniya prirodnyh i prirodno-tekhnogennyh sistem. 2019. S. 101 – 103.
20. Constantin M.J., Owens E.T. Introduction and perspectives of plant genetic and cytogenetic assay. Mutat. Res., M. 99. 1982. R. 1 – 12.
21. Koncevaya I.I., Tolkacheva T.A. Sovershenstvovanie metodiki biotestirovaniya na osnove Alliumtesta. Vesnik Vicebskaga dzyarzhaўnaga ўniversiteta. 2012. T. 6. №72. S. 57 – 65.
22. Pesnya D.S., Romanovskij A.V., Prohorova I.M. Issledovanie toksicheskogo i genotoksicheskih effektov sinteticheskih pishchevyh krasitelej metodom Allium test. YAroslavskij pedagogicheskij vestnik. 2012. T. 3. № 3. S. 86 – 93.
23. Fiskesjö, Geirid Allium screening test. Fiskesjo G. The Allium test as a standard in environmental monitoring, Hereditas. 1985. V. 102. P. 99 – 112. SHveciya: Institut genetiki Lundskogo universiteta, sentyabr' 1989.