English
Русский Sorption characteristics of bacterial cellulose obtained from the symbiotic culture of Medusomyces gisevii
Abstract
The structural and sorption characteristics of bacterial cellulose (BC) synthesized by the symbiotic culture of Kombucha Medusomyces gisevii in nutrient media with different carbon sources (3% sucrose, 6% sucrose, 6% molasses) were studied. It was found that the maximum specific yield of BC biomass was observed when using 6% molasses (11.807 g/g), while the highest content of pure cellulose was recorded in a medium with 6% sucrose (3.81%). Scanning electron microscopy and gas adsorption analysis showed that lyophilized BC samples have a developed macroporous structure. The sorption capacity of the materials with respect to the cationic dye methylene blue was studied. It was found that lyophilized BC possesses a significantly higher sorption capacity (22.809 mg/g) compared to the native film dried by convection (11.689 mg/g). The potential of using bacterial cellulose as a basis for sorption materials and functional carriers is demonstrated.
Objectives: study of the influence of cultivation conditions and drying methods on the structural, morphological and adsorption characteristics of bacterial cellulose.
Methods. Nutrient media with varying carbon sources were used for cultivation. Sample structure was examined using a Nova NanoSem 450 scanning electron microscope (FEI Company, USA) and a TriStar II 3020 gas adsorption analyzer (Micromeritics, USA). Sorption capacity was determined using a Nabi spectrophotometer (MicroDigital Co., Republic of Korea) based on the absorption of methylene blue from an aqueous solution.
Results. Optimal cultivation conditions for maximum biomass yield and cellulose content were determined. It was found that freeze-drying produces a material with a high specific surface area and a developed porous structure, resulting in increased sorption activity.
Conclusions. Bacterial cellulose, especially its lyophilized form, demonstrates high potential as an effective and biocompatible sorbent, as well as a promising carrier of active substances and medicinal preparations.
Objectives: study of the influence of cultivation conditions and drying methods on the structural, morphological and adsorption characteristics of bacterial cellulose.
Methods. Nutrient media with varying carbon sources were used for cultivation. Sample structure was examined using a Nova NanoSem 450 scanning electron microscope (FEI Company, USA) and a TriStar II 3020 gas adsorption analyzer (Micromeritics, USA). Sorption capacity was determined using a Nabi spectrophotometer (MicroDigital Co., Republic of Korea) based on the absorption of methylene blue from an aqueous solution.
Results. Optimal cultivation conditions for maximum biomass yield and cellulose content were determined. It was found that freeze-drying produces a material with a high specific surface area and a developed porous structure, resulting in increased sorption activity.
Conclusions. Bacterial cellulose, especially its lyophilized form, demonstrates high potential as an effective and biocompatible sorbent, as well as a promising carrier of active substances and medicinal preparations.